Hprt mutations in human T-lymphocytes reflect radioprotective effects of the aminothiol, WR-1065
نویسندگان
چکیده
منابع مشابه
Hprt mutations in human T-lymphocytes reflect radioprotective effects of the aminothiol, WR-1065.
Aminothiols such as WR-2721 and its active free thiol WR-1065 have previously been shown to reduce mutations resulting from ionizing radiation in exponentially growing cells. In this study, non-dividing human G0 T-lymphocytes were exposed to the aminothiol radioprotective agent, WR-1065, 30 min before or 3 h after external beam gamma-irradiation and subsequently assessed for survival and mutati...
متن کاملThe aminothiol WR-1065 protects T lymphocytes from ionizing radiation-induced deletions of the HPRT gene.
Aminothiols, such as WR-2721 and its active free thiol, WR-1065, reduce mutations from ionizing radiation in exponentially growing cells. In this study, human noncycling G0 T lymphocytes were exposed in vitro to gamma-irradiation in the presence or absence of WR-1065. The five treatment groups were: (a) control; (b) treatment with 4 mM WR-1065; (c) treatment with 3 Gy of gamma-radiation, from a...
متن کاملFurther evidence that the radioprotective aminothiol, WR-1065, catalytically inactivates mammalian topoisomerase II.
It has recently been proposed that the thiol form of the cytoprotective drug amifostine that is designated WR-1065 [2-((aminopropyl)amino)ethanethiol] exerts its cytoprotective effects in part via a catalytic inhibition of DNA topoisomerase II (topo II)alpha. This in turn leads to the subsequent accumulation of cells in G2 phase and a prolongation of the cell cycle. We have used a Chinese hamst...
متن کاملBinding of the aminothiol WR-1065 to transcription factors influences cellular response to anticancer drugs.
The aminothiol WR-1065 (the active form of amifostine) protects normal tissues from the toxic effects of certain cancer drugs, while leaving their antitumor effects unchanged. The present data address the mechanism of action of this dichotomous effect. (35)S-Labeled WR-1065 bound directly to the transcription factors nuclear factor-kappaB, activator protein-1, and p53, resulting in enhanced bin...
متن کاملThe inhibition of radiation-induced mutagenesis by the combined effects of selenium and the aminothiol WR-1065.
In order to evaluate the anti-mutagenic effects of the potential chemoprotective compounds selenium and (S)-2-(3-aminopropylamino)ethylphosphorothioic acid (WR-1065), CHO AA8 cells were exposed to both compounds either individually or in combination prior to irradiation. Mutation frequency following exposure to 8 Gy was evaluated by quantitation of the mutations detected at the hprt locus of th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Carcinogenesis
سال: 1996
ISSN: 0143-3334,1460-2180
DOI: 10.1093/carcin/17.12.2647